Year: 2016 | Month: April | Volume 9 | Issue 2
Molecular characterization for salinity tolerance in rice using microsatellite markers
Abstract:
A set of six rice cultivars including salt tolerant Narendra Usar Dhan-3 and CSR-30, moderately salt tolerant BPT-5204 and MTU-7029 and salt sensitive Rajendra Bhagwati and Pusa Basmati-1 along with their controlled callus and salt-stressed callus were characterized using a panel of fourteen salt tolerance related microsatellite markers distributed amongst seven chromosomes of rice. The largest amplicon was produced by marker RM242 and the smallest by RM253. A total of 100 allelic products were generated at 22 microsatellite loci with an average of 4.54 alleles per locus. The polymorphism information content values varied from 0.376 (RM4) to 0.827 (RM242) with an average 0.677. A similarity coefficient based dendrogram was constructed using un-weighted paired group method with arithmetic average (UPGMA) for entries classification. The first cluster accommodated salt tolerant cultivars CSR-30, Narendra Usar Dhan-3 and moderately tolerant cultivar MTU-7029, the second salt sensitive cultivar Rajendra Bhagwati, whereas the third moderately salt tolerant cultivar BPT-5204 and salt sensitive cultivar Pusa Basmati-1. Molecular marker based analysis confirmed the induction of salt tolerant calluses in three cultivars MTU-7029, Rajendra Bhagwati and BPT-5204. The work showed the utility of microsatellite markers in ascertaining the status of rice plants with respect to salinity stress tolerance.
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